Technique Creates Functional Platelets from Human Pluripotent Stem Cells

Researchers have genetically manipulated human induced pluripotent stem cells to become stable immortalized lines of megakaryocyte progenitors, which could subsequently produce large quantities of platelets with clotting capabilities similar to those of donated platelets. The technique involved expression of BMI1, BCL-XL, and c-MYC genes to suppress senescence and apoptosis and to promote proliferation. Unlike freshly donated platelets, the immortalized megakaryocyte progenitors could be expanded and frozen for long-term storage. The advance, which is described in a Cell Stem Cell article, could possibly eliminate platelet supply shortages.